The role of phospholipase A_2 in the pathogenesis of endotoxin-induced acute lung injury and the protective effects of chloroquine in rabbits
- VernacularTitle:兔内毒素性急性肺损伤中磷脂酶A_2致伤机制及氯喹保护作用的研究
- Author:
Hong JIANG
;
Jianxin WANG
;
Qingliang XUE
- Publication Type:Journal Article
- Keywords:
respiratory distress syndrome, adult;
endotoxins;
chloroquine;
phospholipase A;
oxidative stress
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the roles of phospholipase A 2 (PLA 2) and oxidative stress in the pathogenesis of acute lung injury(ALI) of rabbits induced by intravascular injection of endotoxin (ET), as well as the protective effects of chloroquine. Methods Rabbits were randomly assigned to three groups: control group, ET group, and ET+chloroquine group(n=8). Acute lung injury was induced by intravascular injection of ET (500?g/kg). The arterial blood gas analysis and serum PLA 2 activity were measured before and after the ET challenge. At the end of the experiment, PLA 2 activity, content of malodialdchyde (MDA), and superoxide dismutase (SOD) in lung tissue were assayed. Electron microscope and light microscope were used to observe the pathological injuries in the pulmonary tissue. Results Compared with saline controls, rabbits treated with ET manifested signs of ALI, such as the decrease of PaO 2(P
