Experimental study of cancer vaccine prepared by fusions of esophageal carcinoma cell line with dendritic cell derived from cord blood
- VernacularTitle:脐血树突状细胞介导的食管癌疫苗实验研究
- Author:
Guanghua GUO
;
Suzuan CHEN
;
Juan ZHANG
- Publication Type:Journal Article
- Keywords:
Dendritic cell Esophageal carcinoma cell line Cell fusion Cancer vaccine
- From:
Chinese Journal of Practical Internal Medicine
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the cancer vaccine (EC109-DC) prepared by fusions of esophageal carcinoma cell line with dendritic cell derived from cord blood and study the biological characteristics and antitumor immunity.Methods CD 34 + hematopoietic stem cells were isolated from cord blood using CD 34 + Progenitor Cell Isolation Kit by magnetic cell sorting system (MACS);expended the cells as DC,and then fused with EC109 by PEG-3600;selected the fusion cells by MACS;observed the phenotypes and proliferation by flow cytometry and technique of cell culture in vitro. Lymphocytes proliferation reaction and the CTL cytotoxicity were examined with MTT.Results EC109-DC could proliferate slowly in vitro and highly expressed CD80,CD83 and CD86.Lymphocytes proliferation reaction and the specific cytotoxicity against EC109 induced by EC109-DC cells were significantly higher than the control groups (P
