Effects of anti-chemokine receptor-5 antibody on type 1 diabetes
- VernacularTitle:抗体封闭趋化蛋白受体5对1型糖尿病发病的影响
- Author:
Hao FENG
;
Baoyu GUO
- Publication Type:Journal Article
- Keywords:
chemokine receptor-5;
diabetes type 1;
T lymphocytes
- From:
Academic Journal of Second Military Medical University
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the anti-diabetogenic effect of antibody blocking portion of chemokine receptor-5 antibody(CCR5) molecule.Methods: The severe combined immunodeficient NOD(NOD.Scid) mice,injected i.p.with splenocytes from nonobese diabetic mouse,were randomly divided into 2 groups(8 of each group): anti-CCR5 Ab group and PBS group.The mice in anti-CCR5 Ab group were treated with a specific polyclonal antibody,targeting the first extracellular loop of CCR5,and PBS group was treated with PBS.Blood glucose levels were measured to observe the anti-diabetogenic effect of the antibody.Histological examination and ELISA analysis were also performed.Results: Within 70 d,all of the control mice developed diabetes while 5 of the 8 mice treated with anti-CCR5 Ab were diabetes-free.The difference of inflammation score between 2 groups was significant(P
