Changes of SHP-2 mRNA of thymus cells of mice with ? ray-induced leukemia
- VernacularTitle:?线诱发的白血病小鼠胸腺细胞SHP-2 mRNA变化的研究
- Author:
Dingde HUANG
;
Qi CHEN
;
Ling HAN
;
Jianmin CAI
- Publication Type:Journal Article
- Keywords:
radiation carcinogenesis;
leukaemia;
signal transduction;
SHP-2;
BALB/c mouse
- From:Journal of Third Military Medical University
2003;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the alterations of SHP-2 mRNA of thymus cells of mice with ? ray-induced leukemia. Methods BALB/c mice were randomly divided into canceration group, non-canceration group, and control group. The gene mutation and content of SHP-2 mRNA in thymus cells were detected by PCR-SSCP, DNA sequencing, and real time fluorescence quantitative PCR (FQ-PCR). Results PCR-SSCP showed there was gene mutation within 700～1 096 bp of SHP-2 mRNA in thymus cells of mice in the canceration group, which was not supported by DNA sequencing. No significant difference in change of SHP-2 mRNA content was found in thymus cells in canceration, non-canceration, and control groups. Conclusion There is translational abnormality of SHP-2 mRNA in thymus cells of mice with ? ray-induced leukemia, which is presented as translational enhancement.
