Inhibitory effect of antisense oligodeoxynucleotide c-myc on the growth of lens epithelial cells induced by galactose
- VernacularTitle:反义c-myc寡核苷酸对半乳糖诱导的晶体上皮细胞生长的抑制作用
- Author:
Fang FAN
;
Zhenglong GE
;
Changfu LI
;
Haixiang LI
;
Xiaopin ZENG
- Publication Type:Journal Article
- Keywords:
c-myc antisense oligodeoxynucleotide;
galactose;
lens epithelial cell
- From:Journal of Third Military Medical University
2003;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of antisense oligodeoxynucleotide c-myc (c-myc ASODN) on the growth of rabbit lens epithelial cells (LECs) induced by galactose. Methods Based on the translation initiation region of the second extron of c-myc gene, oligonucleotides were synthesized and modified with phosphorothioate. Lens epithelial cells were treated with galactose and c-myc ASODN. The effects of c-myc ASODN on the growth of LECs, cultured in high concentration of galactose, were observed by cytometry and MTT. Results The results demonstrated that c-myc ASODN at the doses of 2.5-10.0 ?mol/L inhibited the growth of LECs induced by galactose in a dose-dependent manner. Conclusion These results indicate that c-myc ASODN can inhibit the growth of LEC induced by galactose.
