Study of AFP promoter on the expression of EGFP in human hepatoma cells
- VernacularTitle:FP启动子调控增强型绿色荧光蛋白在人肝癌细胞中表达的研究
- Author:
Ping HAO
;
Donglin WANG
- Publication Type:Journal Article
- Keywords:
enhanced green fluorescent protein;
AFP promoter: transfection;
hepatoma cell
- From:Journal of Third Military Medical University
2003;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of 0.3 kb human alpha-fetoprotein (AFP) promotor on the expression of enhanced green fluorescent protein (EGFP) in human hepatoma cells. Methods By constructing the primer with special enzyme site, human AFP promoter was selectively amplified from human gene bank and eukaryon-expressed pAF-EGFP and pAF-DTA with AFP promotor and EGFP and with AFP promotor and DTA, respectively. pAF-EGFP was transfected into HepG2, SMMC-7721, and NIH3T3. The fluorescence was detected in order to evaluate the transfection rate. Results The expression of EGFP in HepG2 cells transfected with pAF-EGFP was significantly higher than that in SMMC-7721 and NIH3T3 cells (P
