Antioxidative effect of total paeony glycoside on cultured neonatal rat cardiomyocytes injured
- VernacularTitle:赤芍总苷对培养乳鼠心肌细胞损伤的抗氧化作用
- Author:
Xiaoyan MO
;
Haixia HUANG
;
Yu HONG
;
Tao GENG
;
Zhenqiang ZHANG
;
Xiaoyang DU
- Publication Type:Journal Article
- Keywords:
total paeony glycoside;
cardiomyocytes;
antioxidative effect;
superoxide dismutase;
malonaldehyde;
nitric oxide
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the antioxidative effect of total paeony glycoside(TPG) on cardiomyocytes injured.Methods The ischemia and hypoxia injuy model of cultured neonatal rat cardiomyocytes was induced by adding isoprenaline(ISO), and superoxide dismutase(SOD), malonalde hyde(MDA) and nitric oxide(NO) in the culture solution of normal control group; ISO injure group, CoQ 10 positive control group as well as protective group s with high,middle or low-dose TPG were respectively analyzed and compared. Results Compared with normal control group,the enzyme activity o f total SOD, CuZn-SOD and Mn-SOD decreased obviously, and the content of MDA and NO increased markedly in injury group, but in TPG and CoQ 10 groups all of detective indicators had improvement in varying degrees, and the protective effect was better than or close to positive control group in high-dose TPG grou p.Conclusion TPG has protective action on injured cardiomyocyte s induced by ISO in dose-dependent manner. The mechanism relates to the enhance ment of antioxidative effect in cells, and the reduction of membrane damage caus ed by free radical and lipid peroxide.
