Transiently expressed muscle nicotinic acetylcholinereceptors in HEK293 cells
- VernacularTitle:肌肉型乙酰胆碱受体在HEK293细胞上的表达
- Author:
Baozhong YANG
;
Xinliang ZHUANG
;
Xuelian ZHAO
;
Shitong LI
- Publication Type:Journal Article
- Keywords:
muscle acetylcholine receptor;
HEK293 cells;
cell transfec t technique;
receptor clone;
patch-clamp technique
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim Expressing muscle acetylcholin e receptors in mammallian cells using receptor clone technique.Methods The cDNA coding for the ?,?,?, ?,?-subunits of the mouse muscle nAC hR in pSP65, pSP64,pBS SK(-) are subcloned into pcDNA3.1+ by gene recobinant technique and then transfect HEK293 cells using lipofection technique.The ?-n AChR and ?-nAChR are transiently expressed in HEK293 cells membrane. Recording the response of transfected HEK293 cells to acetylcholine by using the whole- cell clamp technique.Results Transfected HEK293 cells may produ ce a inward current as appling acetylcholine.The current values are acctylcholin e-concentration-dependent.Conclusion ?-nAChR or ?-nAChR i s expressed successfully in HEK293 cells.
