FRACTIONATION OF POLYSACCHARIDBS FROM CURCUMA ZEDOARIA ROSC AND ITS BIOACTIVITIES
- VernacularTitle:莪术多糖的分离提取及其生物学活性研究
- Author:
Guanlin WANG
;
Hongmei LUO
;
Hongjun FANG
;
Dan JIANG
;
Hongguang HUANG
- Publication Type:Journal Article
- Keywords:
the polysaccharides of Curcuma zedoaria Rosc;
isolation;
bioactivities
- From:
Acta Nutrimenta Sinica
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To extract the polysaccharides of Curcuma zedoaria Rosc (PCZ) and study its bioactivities. Methods: The crude polysaccharides were extracted from herbal slice of Curcuma zedoaria Rosc with hot water ,and precipitated by ethanol and savage deproteinization and depigmentation with activated charcoal. The constituents were isolated by column chromatography and the total polysaccharides content was determined. Experiments of immune function,anti-oxidation and anti-tumor activity of KM mice and in viro were studied. Results: The content of total polysaccharides of Curcuma zedoaria Rosc was 33.12%. and the extraction rate was 1.938% . It could raise spleen cell proliferation and transformation rate of lymphocyte and SOD activity in blood of mice. A higher antitumor effect of the polysaccharides by injection was observed dose-dependently. Conclusion: PCZ could raise the immune and anti-oxidative activities and then had a higher antitumor effect.
