Interleukin-2? receptor in membrane lipid rafts
- VernacularTitle:T细胞膜脂肪微区域与白细胞介素-2?受体
- Author:
Qiurong LI
;
Jian MA
;
Hao WANG
;
Jieshou LI
- Publication Type:Journal Article
- Keywords:
Lipid rafts;
Interleukin-2? receptor;
Immunoblotting
- From:Parenteral & Enteral Nutrition
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine lipid rafts on the membrane of T cells is the functional microdomains for IL-2? receptor. Methods: Flow cytometric analysis was performed for expression of CD25(IL-2R?) on the surface of T cells. Lipid rafts were isolated by discontinuous sucrose density gradient ultracentrifugation. The localization of IL-2R? in fractions isolated from sucrose density gradients was determined by immunoblotting and detected by chemiluminescence. Results: cells were stimulated with IL-2 and expression of CD25(IL-2R?) on the surface of T cells was 37.08%. Immunoblot analysis of fractions from sucrose gradients revealed that a large proportion of IL-2R? was localized in lipid rafts. Conclusions: lipid rafts is the functional microdomains for IL-2? receptors.
