Combined use of early enteral nutrition, growth hormone and fibrin glue improves healing of intestinal anastomosis in intra-abdominal sepsis rats
- VernacularTitle:早期肠内营养联合生长激素及生物蛋白胶对腹腔感染大鼠肠吻合口愈合的影响
- Author:
Yang BAO
;
Yousheng LI
;
Jieshou LI
- Publication Type:Journal Article
- Keywords:
Intra-abdominal sepsis;
Growth hormone;
Fibrin glue;
Surgical healing;
Enteral nutrition
- From:Parenteral & Enteral Nutrition
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of combined use of early enteral nutrition, growth hormone and fibrin glue on early healing of intestinal anastomosis in intra-abdominal sepsis rats. Methods: Male Wistar rats were randomized into 3 groups. In Control group, antimesenteric transverse enterotomy of 4mm was performed, a second laparotomy was subsequently performed after 24 hours, 6cm of the intestine in the middle of the fistula segment was resected. An end-to-end single layer anastomosis was constructed using 8 interrupted 6-0 polypropylene sutures; In EN group, anastomosis was constructed using 4 interrupted sutures and sprayed fibrin glue, enteral nutrition was given on the second postoperative day for 12 days; In EN/GH group, the animals were treated as EN group, and growth hormone(3.0 U/kg/d) was injected subcutaneously every 24 hours for 14 days. The anastomotic bursting pressure and breaking strength, hydroxyproline content and histology were measured as indicators of wound healing. Results: Anastomotic bursting pressure, anastomotic breaking strength and hydroxyproline content in EN+GH group were higher than that in control and EN groups. Conclusions: Combined use of early enteral nutrition , growth hormone and fibrin glue improves early healing of intestinal anastomosis in intra-abdominal sepsis rats.
