Study of the rdiosensitivity of DL-BSO on rats C_6 glioma cells
- VernacularTitle:DL型丁胱亚磺酰亚胺对大鼠C_6神经胶质瘤细胞放射增敏效应研究
- Author:
Huiqin XU
;
Weizhong ZHAO
- Publication Type:Journal Article
- Keywords:
DL-Buthionine sulfoximine;
rat C 6 glioma cells;
ra diosensitivity
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effect of DL-buthionine s ulfoximine(DL-BSO) on the radiosensitivity of rat C 6 glioma cells under the a erobic and the hypoxic condition. Methods The source of radiati on was 60Co ?-rays. The rats C 6 glioma cells were treated by radia tion alone or DL-BSO+radiation under the aerobic and the hypoxic condition. Col ony forming assay was used to measure effects of DL-BSO on the radiosensitivity . Results Radiosensitive effect of DL-BSO was time-depedent u nder the aerobic condition. After treatment with 0.1 mmol?L -1 DL-BSO fo r 2, 6, 12 hours, the radiosensitive effect was not observed, whereas an enhance ment of radiosensitivity was seen at 24 and 48 hours. An enhancement of radiosen sitivity was seen at 2～48 hours after treatment with 0.1 mmol?L -1 DL-B SO under the hypoxic condition. The radiosensitive effects related to DL-BSO co ncentration under the aerobic and the hypoxic condition. Conclusion Both under the aerobic and the hypoxic conditions DL-BSO can increase the radio sensitivity of rat C 6 glioma cells. DL-BSO increased the rat C 6 gliom a cells radiosensitivity especially under the hypoxic condition, and radiosensit ive effect of DL-BSO is time and concentration-dependent.
