Change in cytosolic Ca~(2+) in smooth muscle cells in gastric antrum of diabetic rats
- VernacularTitle:糖尿病大鼠胃窦平滑肌细胞内钙离子的变化及意义
- Author:
Qinglin LONG
;
Dianchun FANG
;
Hongtao SHI
- Publication Type:Journal Article
- Keywords:
Ca 2+;
diabetes mellitus;
stomach;
smooth muscle
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the change in cytosolic Ca 2+ in smooth muscle cells in gastric antrum of diabetic rat. Methods Rats were randomly divided into diabetic group and control group. Gastric empty time was determined 3 months after diabetes was reproduced in rats, the apoptosis rate of smooth muscle cells in the gastric antrum was assessed by flow cytometry, and the content of cytosolic Ca 2+ in smooth muscle cells was measured by laser scanning confocal microscopy. Results In diabetic rats, gastric emptying was significantly delayed. Compared the normal rats, the content of cytosolic Ca 2+ in smooth muscle cells from the antrum of diabetic rats was increased. The apoptosis rate of smooth muscle cells in the antrum of diabetic rats was 15%, and it was higher than that in normal rats. Conclusion With the increase in the content of cytosolic Ca 2+ in smooth muscle cells, smooth muscles were damaged and therefore their contraction was also impaired.
