Effect of furosemide on Cl -/HCO_3~- exchange in IMCD cell in rabbit kidney
- VernacularTitle:速尿对家兔肾脏内髓集合管细胞Cl~-/HCO_3~-交换的影响
- Author:
Qianming XIA
;
Yan QUAN
;
Hongyan LI
- Publication Type:Journal Article
- Keywords:
furosemide;
kidney tubules,collecting;
inner medullary collecting duct;
Cl -/HCO- 3 exchange
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of furosemide on Cl -/HCO- 3 exchange of inner medullary collecting duct(IMCD) in rabbit kidney. Methods The effect of furosemide in different concentrations on the changes in Cl -/HCO- 3 exchange in mono-layer of IMCD cell in rabbit kidney was determined by fluorescent probe technique. Results Cl -/HCO- 3 exchange in IMCD cell could be inhibited by 4.3% by 15?mol/L furo semide solution, and 480?mol/L furosemide solution could inhibit the exchange by 97.4%. The Cl -/HCO- 3 exchange rates of the groups, in which the final concentrations of furosemide were equal to or higher than 30?mol/L, were significantly lower than that of the control group(P
