Inhibitory effects and mechanism of arsenic trioxide on the growth of melanoma B16 cells
- VernacularTitle:三氧化二砷抑制小鼠B16黑色素瘤生长作用及其机制
- Author:
Jun XIA
;
Junxia CHEN
;
Lihua YU
;
Xiuyun CUI
;
Zhiwe CHEN
- Publication Type:Journal Article
- Keywords:
arsenic trioxide;
B16 cell;
angiogenesis;
anti-proliferative effect;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim The present study was designed to investigate the growth inhibition and anti-angiogenesis of arsenic trioxide (As 2O 3) on B16 cells xenografts in C57BL/6J mice and observe the effects of As 2O 3 on cell proliferation, cell morphology, cell cycle and apoptosis in vitro. Methods Mice melanoma cell line B16 was transplanted into C57BL/6J mice. The weight of tumor and percent of tumor development were examined after As 2O 3 intraperitoneal administration. HE staining and immunohistochemistry for Ⅷ-R Ag were performed to detect the microvessel density in tumor tissues. CellTiter 96 Aqueous One was used to determine the cell proliferation. Giemsa and Feulgen staining were used to observe the morphological changes of the cells. Cell cycle and apoptosis were analyzed by flow cytometry (FCM). Results As 2O 3 significantly inhibited the tumor growth in vivo, with an inhibitory rate of 81.61%. The microvessel density in tumor tissues was obviously reduced after treated with As 2O 3. There was obviously a concentration-dependent relationship between As 2O 3 and the inhibition of B16 cells proliferation (P
