Relationships between induction of apoptosis by Taxol in Hela cells and apoptosis-related proteins
- VernacularTitle:紫杉醇诱导Hela细胞凋亡及其与凋亡相关蛋白的关系
- Author:
Xiangyang HU
;
Gang MENG
;
Yangyi BAO
;
Xiaomei ZHU
;
Yuan WANG
;
Qin ZHOU
- Publication Type:Journal Article
- Keywords:
Taxol;
Hela;
PCNA;
caspase-3;
apoptosis
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of taxol on apoptosis in Hela cell and the mechanism of apoptosis. The apoptotic cells were detected by TUNEL, HE, eletronic micrpscopy and flow cytometry method. The expressions and activity of apoptosis associated proteins such as PCNA and caspase-3 were examined using S-P and enzyme histochemistric method.The results followed as: HeLa cells exposed to Taxol undergo cell death, presenting morphological and biochemical characteristics typical of apoptosis and the apoptotic cells increased with time and concentration. In contrast to untreated Hela cells, which express low PCNA, Ones treated with Taxol expressed high amounts of PCNA. Conclusion Taxol may induced apoptosis in Hela cell. The apoptosis induced by taxol is related to the increase of PCNA protein and activity of caspase-3.
