Effects of genistein on expression of nuclear factor-?B and levels of tumor necrosis factor-? in asthma patients
- VernacularTitle:染料木黄酮对哮喘患者核因子-?B表达和肿瘤坏死因子-?分泌的影响
- Author:
Xingyu GU
;
Xiaoju LIU
- Publication Type:Journal Article
- Keywords:
genistein;
asthma;
nuclear factor-?B;
tumor necrosis factor-?
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of genistein on the expression of nuclear factor-?B (NF-?B) and the levels of tumor necrosis factor-? (TNF-?) in peripheral blood mononuclear cells (PBMCs) of asthma patients. METHODS: PBMCs were isolated from blood samples of 32 asthma patients and 31 healthy persons, which was divided into control groups, dexamethasone-treated groups and genistein-treated groups. The expression of NF-?B was analyzed by immunocytochemical staining. The level of TNF-? was measured by radioimmunoassay. RESULTS: The percentage of NF-?B-positive cells in PBMCs and the level of TNF-? in PBMCs culture supernatants were significantly higher in asthma patients than in healthy persons (all P
