Effect of pancreatic elastase on expression of TNF-? and IL-1? in Kupffer cells induced by lipopolysacchride
- VernacularTitle:胰弹性蛋白酶对内毒素诱导的肝枯否细胞TNF-?、IL-1?表达的影响
- Author:
Dongxuan LI
;
Fuzhou TIAN
;
Hon LI
- Publication Type:Journal Article
- Keywords:
acute pancreatitis;
hepatic Kupffer cell;
systemic inflammatory response syndrome;
TNF-?;
IL-1?
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of pancreatic elastase on the expression of TNF ? and IL 1? in Kupffer cells induced by lipopolysacchride. Method Cultivating Kupffer cells were divided into 3 groups. In group A, physiological saline was added into the culture medium as control (control group). Lipopolysacchride (LPS) was added instead of saline in group B (LPS group). In group C both lipopolysacchride and pancreatic elastase were added to the cultere medium (LPS+elastase group). The expressions of TNF ?, IL 1? and TLR4 mRNA in Kupffer cells were determined by RT PCR, and concentrations of TNF ? and IL 1? in the culture media by ELISA. Results The results of both RT PCR and ELISA indicated that the expressions of TNF ? and IL 1? in group C was significantly higher than that in group B. Conclusion It was concluded that pancreatic proteases such as elastase could enhance the expressions of TNF ? and IL 1? of hepatic Kupffer cells induced by lipopolysacchride, and the results the might offer the explanation why inflammatory reaction could be amplified in the course of acute pancreatitis
