Influence of tretinoin on neural specific gene expression in fetal liver cells and its signaling pathways
- VernacularTitle:维A酸对人胎肝细胞神经组织特异基因表达的影响及其信号途径
- Author:
Gexiu LIU
;
Huan ZHANG
;
Dongmei HE
- Publication Type:Journal Article
- Keywords:
tretinoin;
embryo;
liver cells;
neural specific genes;
protein kinase C
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanism of tretinoin-induced neural differentiation in fetal liver CD34+ cells in vitro. Methods :CD34+ cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C(PKC) inhibitor chelerythrine chloride(3 ?mol/L) were induced by 5?10-7mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR. Results: Tretinoin significantly promoted expression of neural specific genes such as Nestin, neuron-specific nuclear protein (NeuN) , neuron-specific neuronfilament-M (NF-M) , and MAP-2 in fetal liver CD34+ cells, with Nestin increased by 4. 09 folds, NeuN by 5. 12 folds, and NF-M by 7. 27 folds (all P
