Effects of different zinc intakes on brain and testis expression of zinc transporter 3 mRNA in weaned mice
- VernacularTitle:不同饲料锌水平对哺乳期后幼鼠大脑及睾丸组织ZnT3mRNA表达的影响
- Author:
Yanqin ZHANG
;
Jiangang LONG
;
Hui SHEN
;
Haihong QIN
;
Fudi WANG
- Publication Type:Journal Article
- Keywords:
zinc;
zinc transporter 3;
brain;
testis;
post-weaning
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the growth and development of the weaned mice fed with different levels of dietary zinc and to explore the expression of zinc transporter 3(ZnT3) mRNA induced by different dietary zinc intakes. Methods: Twenty male weaned mice (postnatal day 21) were divided into 4 groups: zinc deficient (ZD), zinc adequate(ZA), zinc supplemental (ZS) and pair fed(PF). Mice were fed with different levels of dietary zinc for 3 weeks (from postnatal day 21 to postnatal day 42) ;the zinc contents of ZD, ZA, ZS and PF group were 1 mg/kg, 30 mg/kg, 180 mg/kg and 180 mg/kg respectively. From postnatal day 21 to postnatal day 42, the diet intakes and weight of the mice were measured everyday. On postnatal day 42, the mice were sacrificed and tissues were immediately isolated and frozen lor RNA extraction. The serum zinc concentrations were measured by AAS and the expression of ZnT3 mRNA was determined by semiquantitative RT-PCR. Results: The dietary intakes and weight of ZD mice were much lower than that of other groups(P3
