Potential effect and mechanism of IFN? on tumor angiogenesis
- VernacularTitle:IFN?在抗肿瘤新生血管形成中潜在的作用及机制研究
- Author:
Qizhen CAO
;
Zhibin LIN
- Publication Type:Journal Article
- Keywords:
IFN?;
tumor angiogenesis;
HUVEC;
human lung carcinoma cell PG
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect and potential mechanism of IFN? on tumor angiogenesis. METHODS: Human umbilical cord vascular endothelial cell (HUVEC) was cultured in vitro. The effect of IFN? (10, 100, and 1000 unit?ml -1) on proliferation of HUVEC was detected by MTT assay in vitro. HUVEC pretreated with IFN? (10, 100, and 1000 unit?ml -1) was stained with PI and detected by flow cytometry (FCM). The mRNA expression of apoptosis relative gene Bcl-2 and Bax of HUVEC treated with IFN? (100, and 1 000 unit?ml -1) were detected with semi quantitative reverse transcription- polymerase chain reaction (RT-PCR). IFN? (30, 300, and 3 000 unit?ml -1) on VEGF secretion and expression in human lung carcinoma cell PG in hypoxia were detected by ELISA assay. CONCLUSION: The key attributes of IFN? on tumor angiogenesis possible directly inhibit vascular endothelial cells or indirectly inhibit growth factors secretion and expression in tumor cells.
