Effects of puerarin on GLUT4 in adipocyte of rats with insulin resistance
- VernacularTitle:葛根素对胰岛素抵抗大鼠脂肪细胞葡萄糖转运蛋白4的影响
- Author:
Juanjuan LI
;
Huimin BI
- Publication Type:Journal Article
- Keywords:
puerarin;
GLUT4;
insulin resistance;
adipocyte;
animal models
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of puerarin on expression and translocation of glucose transporter 4(GLUT4) in adipocyte of rats with insulin resistance induced by diets. METHODS: Experimental rats were divided into three groups: control group (n=10), model group (n=10) and puerarin group (n=10) randomly. Animals in model group and puerarin group were fed with the diets enriched with sucrose (20%, w/w), lard (10%, w/w), cholesterol( 2.5%, w/w) and cholic acid (1%, w/w) to induce insulin resistance. Puerarin group were treated with puerarin (100 mg?kg -1 body weight, ip) every day for 6 weeks. After the plasma membranes and the intracellular membranes of adipocyte were prepared and separated, GLUT4 protein was detected by Western blot analysis. Body weight, serum triglyceride and cholesterol, fasting plasma glucose and serum insulin concentration were detected termly. And the insulin sensitivity index was also calculated. RESULTS: Western blot analysis showed that intracellular membranes GLUT4 protein in adipocyte of model group rats decreased by 38.72% (P
