Protective effect of baicalin-mediated on mice spinal ganglion cells against gentamicin-induced ototoxicity
- VernacularTitle:黄芩苷对庆大霉素致耳蜗螺旋神经节细胞损伤作用的影响
- Author:
De DAI
- Publication Type:Journal Article
- Keywords:
baicalin;
gentamicin;
spinal ganglion;
free radical;
lipid peroxidation
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the protective effect of baicalin (BA) against the ototoxicity of gentamicin (GM) of mice spinal ganglion (SG) cells. METHODS: MTT assay was employed to determine the growth inhibition of GM on the SG cells. The activities of superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-PX), and the content of malondialdehyde (MDA) were measured by UV chromatography in SG cells from mice of different groups. RESULTS:Baicalin reversibly limited the GM-induced growth inhibition of SG cells in a dose-dependent manner. BA increased the activity of T-SOD and GM-induced decreased activity of T-SOD and GSH-PX in SG cells. The compound could also reduce MDA level and limite the GM-induced high content of MDA in SG cells. CONCLUSION: Baicalin mediated neuroprotective effect on SG cells is against gentamicin-induced ototoxicity through anti-oxidant.
