EFFECT OF DIETARY NUCLEOTIDES ON DNA DAMAGE OF THYMOCYTES IN MICE
- VernacularTitle:外源核苷酸对免疫抑制小鼠胸腺细胞DNA损伤的影响
- Author:
Lanfang WANG
;
Guowei LE
;
Yonghui SHI
;
Tiesheng WANG
- Publication Type:Journal Article
- Keywords:
nucleotides;
DNA damage;
SCGE;
cyclophosphamide
- From:
Acta Nutrimenta Sinica
1956;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of dietary nucleotides on DNA damage of thymocytes in mice. Methods: KM mice (n=30) , 5-6 weeks, were randomized into 3 groups,negative group (group 1), positive group (group 2) and nucleotides group (group 3). Mice in group 1 and 2 were fed nucleotide-free diet and group 3 nucleotide-supplemented diet (0.25% nucleotides). Mice in group 2, 3 were given single cyclophosphamide intraperitoneal injection of 150 mg/kg bw at day 21. DNA damage in thymus lymphocytes was evaluated 18 h after injection by single-cell gel electrophoresis (SCGE) assay. Thymus and spleen were weighed. Results: Dietary nucleotides had no effect on weights of thymus and spleen, but significantly decreased the number of damaged lymphocytes and the degree of damage. Conclusion: Dietary nucleotides may protect thymocytes DNA in mice from damage.
