EFFECT OF GRAPE PROCYANIDIN ON N-NITRO COMPOUNDS-INDUCED EXPRESSION OF TNF-?mRNA AND TGF-?_1 mRNA IN THE HEPATIC CELLS OF RATS
- VernacularTitle:葡多酚对大鼠肝脏TNF-?与TGF-?_1mRNA异常表达的抑制作用
- Author:
Hongqun CONG
;
Jinyi ZHONG
- Publication Type:Journal Article
- Keywords:
grape procyanidin;
hepatic cell;
TNF-;
TGF-?1;
N-nitroso compounds
- From:
Acta Nutrimenta Sinica
1956;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of grape procyanidin (GPC) on N-nitroso compounds-induced expression of TNF- mRNA and TGF-?1 mRNA in hepatic cells. Methods: NaNO2 was infused into Wistar rats by 50 mg/kg bw to induce mutation. GPC was infused by 100mg/kg bw and 10mg/kg bw respectively through mouth into the rats of two experimental groups. After 8 w situ hybridization was applied to measure the expression level of TNF-?mRNA and TGF-?1 mRNA in hepatic cells. Results: The expression rate of TNF-醡RNA and TGF-?1 mRNA of the hepatic cells in positive control group was 30.23 % and 19.47 % respectively, while that of high-dose GPC group was 11.94 % and 6.96 % respectively .The differences were statistically significant (P
