Experimental study of differentiation of bone marrow mesenchymal stem cells to epidermal cells and vascular endothelial cells under the induction of serum from rat with burn injury
- VernacularTitle:烧伤大鼠血清诱导骨髓间充质干细胞分化为表皮细胞和血管内皮细胞的实验研究
- Author:
Bing HAN
;
Xiaobing FU
;
Xuemei LIANG
- Publication Type:Journal Article
- Keywords:
cell differentiation;
endothelium, vascular;
cell,dermal;
bone mesenchymal stem cells;
burns
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of serum from rats with burn injury on the differentiation of bone marrow mesenchymal stem cells(MSCs). Methods Bone marrow was extracted from sacrificed Wistar rats and MSCs were then incubated in F-12 medium in the presence of fetal calf serum (FCS) (group F),normal rat serum (group N),or serum harvested from rats with burn injury(group B). Cells of the fifth passage were harvested to investigate the expression of CK and FⅧ by immunohistochemistry and flow cytometry. Results With immunohistochemical staining,both CK and FⅧ were positive in MSCs of group B and negative in the cells of the other two groups. The flow cytometry analysis showed that the positive rates of CK and FⅧ staining MSCs of group B were higher than that of the other two groups ( P 0.05). Conclusions The serum obtained from rats with burn injury could induce differentiation of MSCs to both epithelial cells and vascular endothelial cells,implying that serum after barm injury might promote the MSCs to participate in the process of wound repair.
