A study of cytotoxicity of membrane material of cardiac assist pump
- VernacularTitle:心脏辅助泵隔膜材料聚氨酯的细胞毒性研究
- Author:
Jincheng LIU
;
Dinghua YI
;
Jian YANG
- Publication Type:Journal Article
- Keywords:
polyurethanes;
cytotoxicity;
toxicology;
heart-assist devices
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the cytotoxicity of the membrane material of heart assist pump. Methods The membrane materials tested incladed polyurethane (group A) and medical polychloroethylene (group B),and a blank control group with none of the material. L-929 cells (the mouse fibroblasts) were cultured on either material in groups A and B,and 6 cell cultures were without any material. After 24,48 and 72 hours,the growth of cell in groups A,B and the control was observed,and the number was counted. Educts of two materials used in groups A and B was respectively added to culture medium,then the OD was measured every 24h and proliferation rate was determined by MTT test. Results L-929 cells grew very well in groups A and B with less death. The cell counts showed no difference between 2 groups at each time. The OD of group A was always higher than that of group B after 24,48 and 72h ( P
