A study of localization of NMDA receptor protein labelling with colloid-gold on membrane by Scanning Electron Microscopy
- VernacularTitle:神经细胞膜NMDA受体蛋白单分子胶体金标记扫描电镜定位研究
- Author:
Yigang YU
;
Ruxiang XU
;
Xiaodan JIANG
- Publication Type:Journal Article
- Keywords:
neuron;
receptor,NMDA;
immunity colloidal gold technique;
field emission SEM;
lipid rafts
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution rule of NMDA receptor protein localizated on neuron membrane surface. Methods After the primary culture model of forebrain cortex neuron of rat had been set up,the NR1 subunit protein molecule of NMDA receptor has been combined with primary antibody IgG,and marked by Staphylococcus protein A-colloid gold particles (SPA-G). Then the neuron was observed with Field-emission SEM,and the content of AU element was calcalated by software. Results The cluster of NMDA receptor combined with SPA-G particulates was clearly observed as some globular being distributed mainly under some niches of lipid rafts,mainly localized at originating section of dendritic protuberance of neuron membrane surface. Conclusion NMDA receptor molecule protein was distributed on the membrane surface lipid rafts of originating section of dendritic protuberance of neuron. It was in accord with the distribution rule of postsynaptic membrane on neuron. The Field-emission SEM could distinguish single NMDA receptor melocule cluster after being marked with SPA-G molecule in neuron.
