Promoting effects of interleukin 13on proliferation of murine airway smooth muscle cells
- VernacularTitle:IL-13对小鼠气道平滑肌细胞的促增殖作用
- Author:
Weijun CAO
;
Qiang LI
;
Zhongling LIU
- Publication Type:Journal Article
- Keywords:
airway smooth muscle cell;
cell proliferation;
interleukin-13;
epidermal growth factor receptor
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the promoting effects of interleukin-13(IL-13)on the proliferation of airway smooth muscle cells(ASMC)in mice and its mechanism.Methods:Murine ASMC were isolated and subcultured.IL-13,IL-13plus AG1478,IL-13plus neutralizing antibody to TGF-?,IL-13plus neutralizing antibody to EGF,IL-13plus neutralizing antibody to HB-EGF,IL-13plus dexamethasone were added to the media,respectively.MTT and 3 H-TdR incorporation assays were used.Apical media were analyzed for the presence of soluble TGF-?using ELISA.Results:The D 595 value of MTT assay and the cpm value of 3 H-TdR incorporation assay of IL-13group were significantly higher than those of control group(P
