Effects of repeated treatment with different concentrations of chemotherapy drugs on proliferation and chemo-resistance in human lung cancer cell line
- VernacularTitle:不同浓度常用化疗药物连续刺激对人肺腺癌细胞株增殖耐药产生的影响
- Author:
Zhihong CHI
;
Peng LI
;
Duanqi LIU
;
Jiren ZHANG
;
Ra SHI
- Publication Type:Journal Article
- Keywords:
lung neoplasms;
flow cytometry;
cell proliferation;
drug resistance,neoplasm
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the variation of tumor cell proteins correlated with cell proliferation and chemo-resis-tance after repeated treatment with chemotherapy drugs.Methods:The human lung cancer cell line(LPET-a-2)was repeat-edly treated with chemotherapy drugs:doxorubicin,etoposide,cisplatin and the combination of the3drugs,and each drug was given at2concentrations.The treatment intervals were recorded.ErbB-2,c-myc,MDR1,MRP,LRP,ref-1and NF-?B were tested by flow cytometry.The cells expressing each protein,the mean and total quantity of each protein after each treat-ment were calculated by different drugs at different concentrations.Results:The levels of every protein decreased along with the time of culture.In high-dose group,every item decreased along with the time of treatment.In low-dose group,there was no rule for the item variation with some decreased and some increased.Conclusion:Low-dose anticancer drugs are easier to induce cell proliferation and chemo-resistance than high-dose one,which suggests that adequate chemotherapy should be given in clinical practice.
