Dehydroepiandrosterone and 7-oxo-dehydroepiandrosterone (7-oxo-DHEA) protected hippocampal neurons against neurotoxicity induced by glutamate
- VernacularTitle:脱氢表雄酮及其衍生物对谷氨酸致海马神经细胞损伤的保护作用
- Author:
Nan YANG
;
Yanyong LIU
;
Pingping ZUO
- Publication Type:Journal Article
- Keywords:
DHEA;
7-oxo-DHEA;
hippocampal neurons;
glutamate;
celluar Ca 2+
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study Dehydroepiandrosterone (DHEA) and 7-oxo-dehydroepiandrosterone (7-oxo-DHEA) protected hippocampal neurons against neurotoxicity induced by glutamate(Glu). Methods Cell survival rate was analyzed using MTT colorimetry, the change of Ca 2+ levels and the levels of free radical in cultured hippocampal neurons were analyzed by the laser scanning confocal microscope, and cellular GSH level was also analyzed. Results Exposure of cultured rat hippocampal neurons to Glu resulted in accumulation of celluar Ca 2+ and cellular free radical were prevented by DHEA (0.1 ?mol?L -1 )and 7-oxo-DHEA(0.1 ?mol?L -1),and cellular GSH was increased. Conclusion DHEA and 7-oxo-DHEA protected hippocampal neurons against neurotoxicity induced by Glu.
