Inhibitory effects of sinomenine on the activation of nuclear factor-?B and on the degradation of I?B
- VernacularTitle:青藤碱对核转录因子?B及其抑制因子I?B的影响
- Author:
Xiaokun JIN
;
Weidong LI
;
Huiling TENG
;
Zhibin LIN
- Publication Type:Journal Article
- Keywords:
sinomenine(SIN);
rheumatoid arthritis(RA);
peritoneal macrophages;
nuclear transcription factors-?B;
I?B
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effect of Sinomenine on the activation of NF-?B and the degradation of its inhibitor I-?B in peritoneal macrophages of BALB/c mice in vitro and provide experimental evidence for further evaluation of the antiinflammatory and antirheumatic effects of Sinomenine. Methods Effect of Sinomenine on the activation of NF-?B p65 in the cells was investigated by using fluorescence-labelling and laser confocal scanning microscopy; Effect of Sinomenine on the degradation of I?B-? was investigated by Western blot. Results SIN (0.25,1.25 mmol?L -1) attenuated the activation of NF-?B p65 in peritoneal macrophages of BALB/c mice induced by LPS in vitro. SIN(0.25,1.25 mmol?L -1) inhibited the degradation of I-?B-? in peritoneal macrophages of BALB/c mice induced by LPS in vitro. Conclusion SIN can partially inhibit the activation of NF-?B p65 and the degradation of I?B-? in peritoneal macrophages of BALB/c mice induced by LPS in vitro.
