Effect of saikogenin d on prostaglandin E_2 (PGE_2) production in vitro in C_6 rat glioma cells
- VernacularTitle:体外柴胡皂苷元d对C_6大鼠神经胶质瘤细胞前列腺素E_2生成的影响
- Author:
Xiaochuan LV
;
Lin BAI
;
Xiaolei WANG
- Publication Type:Journal Article
- Keywords:
saikogenin d;
prostaglandin E 2;
arachidonic acid;
cyclooxygenase;
C 6 rat glioma cells
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of saikogenin d (SGD) on prostaglandin E 2(PGE 2) production in C 6 rat glioma cells. Methods Radioimmunoassay was applied to determine PGE 2 production in the cells. Scintillation counting was used to measure liberation of -arachidonic acid (AA) from the cells labeled with -AA. Results In vitro, SGD alone at 1～20 ?mol?L -1 did not affect PGE 2 release from cells, but inhibited its release induced by A23187, a Ca 2+ ionophore. The inhibition was concentration-dependent, with the IC 50 value of about 3 ?mol?L -1. SGD (2～10 ?mol?L -1 ) had no inhibitory effect on A23187-induced AA release or on the conversion of AA to PGE 2 in microsomal preparations. Conclusion SGD inhibites A23187-induced PGE 2 production in C 6 rat glioma cells in vitro, without either inhibition of free AA liberation or a direct inhibition of cyclooxygenase (COX) activity.
