Detection of gene mutations with PCR and anti-colon cancer monoclonal antibody in tissue and stool of patients with colorectal cancer
- VernacularTitle:应用PCR和单克隆抗体方法从人粪便及肿瘤组织中检测大肠癌基因突变的研究
- Author:
Qizhen CAO
;
Gang NIU
;
Liyong GAO
;
Zifen GAO
;
Huanran TAN
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
K-ras gene;
gene mutation;
colorectal cancer monoclone antibody;
DNA from stool
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To detect sequence and mutation of K-ras oncogene in tissue and stool DNA of patients with colorectal cancer in order to provide a method of noninvasive and simple colorectal cancer diagnosis. METHODS: DNA was separated and purified from colorectal cancer tissue or stool of patient with colorectal cancer, then the K-ras gene was amplified by PCR and PCR products were cloned, the K-ras gene was sequenced, and the mutation was identified. The expression of color/colorectal cancer antigen was inspected by immunohistochemical technique. Stool sample of patient with colorectal cancer was detected with enzyme-linked immunosorbent assay (ELISA). RESULTS: K-ras gene sequence of the stool was completely same as that of the tissue of the patient;K-ras mutation was detected in one case. There was relativity between the mutation of K-ras gene and the pathology type of colorectal cancer and the expression level of colorectal cancer antigen in stool sample. CONCLUSION: It is feasible that colorectal tumors can be detected by a noninvasive method based on the molecular pathogenesis of the disease. Detecting K-ras gene mutations of stool DNA can provide bases for the screening, early detection, and prognosis to patients with colorectal cancer.
