Expressions of c-fos and c-myc mediated by ? 1-adrenergic receptor in rat ventricular muscles
- VernacularTitle:大鼠心室肌?_1-肾上腺素能受体介导的c-fos、c-myc表达
- Author:
Xiaohong LIU
;
Zhenyu WEI
;
Yuan GAO
- Publication Type:Journal Article
- Keywords:
norepinephrine;
? 1-adrenergic receptor;
c-fos;
c-myc;
signal transduction;
rat
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the signal transduction pathway of norepinephrine (NE)-induced expressions of oncogenes c-fos and c-myc in the rat heart. Methods mRNA levels of c-fos and c-myc in the left ventricle of the Langendorff-perfused rat heart was quantitated by reverse transcription and polymerase chain reaction (RT-PCR). Results NE increased the expressions of c-fos and c-myc while the effects of NE on the expressions of c-fos and c-myc were blocked by ? 1-adrenergic blocker prazosin (5?10 -6 mol/L) completely. Neomycin (5 mmol/L) and ruthenium red (10 ?mol/L) decreased the NE-induced c-fos and c-myc mRNA levels. Conclusion NE can induce the expressions of oncogenes c-fos and c-myc in the ventricle mediated by ? 1-adrenergic receptor. The signal transduction pathway of NE-induced c-fos and c-myc expressions may be associated with inositol phosphates-Ca 2+ system and diacylglycerol-protein kinase C system.
