The cellular immune responses induced by Mycobacterium tuberculosis H37Ra in mice
- VernacularTitle:结核菌H37Ra在小鼠体内诱导的抗结核细胞免疫应答的研究
- Author:
Shuling WANG
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
H37Ra;
Cellular immune response
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the cellular immune responses induced by Mycobacterium tuberculosis H37Ra and examine its protective efficacy against MTB in mice.Methods:Balb/c mice were divided into H37Ra,BCG and NS groups randomly,and intracutaneously injected at caudal region with H37Ra,BCG and NS respectively.At 8 weeks after immunization,some mice were sacrificed,and the percentages of CD3~+T,CD4~+T and CD8~+T-lymphocyte in spleen were detected by flow cytometry.The spleen lymphocytes of mice were cultured with PPD in vitro;the proliferation of splenocytes was detected by MTT assay.The productions of IFN-?in the supematant of culture were determined by ELISA.After 8 weeks of immunization,Balb/c mice were challenged via intraperitoneal route with MTB H37Rv strain.Four weeks later,mice were sacrificed,then the bacteria load in the lung were determined.Results:After 8 weeks of immunization,the percentages of CD3~+T and CD4~+T-lymphocyte in mice of H37Ra vaccination were significantly higher than those of the NS control subjects(41.63?1.68)%,(27.08?0.58)% vs (38.34?0.74)%,(24.37?1.60)%(P0.05).The proliferation of antigen-specific lymphocytes,the productions of IFN-?in the H37Ra group were significantly higher than those of the NS group(P0.05).At 4 weeks after challenge,compared with the NS group, the mean numbers of viable bacteria in the lung of the H37Ra group reduced 0.954log_(10)CFU(P0.05).Conclusion:Mycobacterium tuberculosis H37Ra could induce specific cellular immune responses in mice.The immunity effect of H37Ra is similar to BCG.
