Study on mechanisms of intervention of Fufangbiejiaruanganfang drug serum to cell cycle of hepatic stellate cells
- VernacularTitle:复方鳖甲软肝方对肝星状细胞细胞周期的影响
- Author:
Shungen GUO
;
Min DAI
;
Yanshuang XIE
- Publication Type:Journal Article
- Keywords:
receptors, transforming growth factor beta;
Fufangbiejiaruanganfang drug serum;
hepatic stellate cells;
cell cycle;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of the serum containing Fufangbiejiaruanganfang (FFBJRGF) on cell cycle and apoptosis of hepatic stellate cells(HSC). Methods The influence of TGF-? 1 on cell cycle and rate of apoptosis of HSC in vitro was examined by using flow cytometer, furthermore, the effects of FFBJRGF drug sera on HSC were observed compared with IFN-? serum and normal rat serum controls. Results The numbers in phase of G 0 and G 1 were increased when HSCs were incubated by adding TGF-? 1, IFN-? serum and different dosages of FFBJRGF drug serum respectively, especially being predominant in the FFBJRGP groups. Meanwhile, the intervenient roles in phase of G 2?M?S of HSC were found in FFBJRGF drug serum treatments. Compared with normal serum control group, there were not notable effects on the cellular apoptosis of HSC in the different dosages of FFBJRGF drug serum groups as well as IFN-? group, however, TGF-? 1 could significantly increased HSC apoptosis rate. Conclusion Our results indicates FFBJRGF has anti-hepatic fibrosis efficiency through inhabiting the proliferative cycle of HSC, not up-regulating HSC apoptosis in vitro.
