Effects of valsartan on the expression of aging related gene at different segments of nephron
- VernacularTitle:缬沙坦对肾单位不同节段衰老相关基因表达的影响
- Author:
Li ZHANG
;
Xiangmei CHEN
;
Hang LIU
- Publication Type:Journal Article
- Keywords:
aging;
renin-angiotensin system;
extracellular matrix;
transforming growth factor beta;
fibronectin
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of chronic angiotensin Ⅱ blockade on gene expression of extracellular matrix at different segments of nephron in aged rats. Methods Glomeruli and tubules of rat kidney were isolated by laser microdissection and pressure catapulting system. Gene expression level of transforming growth factor (TGF-? 1) and fibronectin (FN) were assessed. Fifteen -month-old male Wistar rats were treated with the valsartan (25mg/kg) until sacrificed at age 24 months, and their TGF-? 1 and FN expression levels were compared with that of young and aged controls. Results Compared with young rats, TGF-? 1 mRNA and FN mRNA expression were both up-regulated in isolated glomeruli and tubules in aged rats. Although Valsartan had no effect on the gene expression of TGF-? 1 in isolated glomeruli, it down regulated TGF-? 1 mRNA in tubules of aged rats. Valsartan also down regulated FN mRNA in both glomeruli and tubules of aged rats. Conclusion The results indicate that changes in TGF-? 1 and FN expression might be involved in the aging related changes of gomeruli and tubules. Activation of RAS at the different segments of nephron in aged rats might regulate the gene expression of ECM through diverse mechanisms.
