Effects of hydrogen peroxide on intracellular free calcium concentration in cultured neonatal rat cardiac myocytes and its antagonism by carvedilol
- VernacularTitle:过氧化氢对乳鼠心肌细胞内游离钙浓度的影响及卡维地洛的拮抗作用
- Author:
Hexiang CHENG
;
Rongqing ZHANG
;
Guoliang JIA
- Publication Type:Journal Article
- Keywords:
cardiocyte;
hydrogen peroxide;
calcium;
carvedilol
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine effects of hydrogen peroxide on intracellular free calcium concentration(i) in cardiac myocytes and its antagonism by carvedilol. Methods A cell culture of neonatal rat cardiac myocytes was used for experimentation. They were divided into four groups, i.e. control group, hydrogen peroxide (H 2O 2) group, carvedilol group,and H 2O 2 + carvedilol group. Confocal microscope was used with Fluo-3/AM as calcium indicator to detect changes in i immediately and 15 minutes after H 2O 2 intervention, respectively. Results The intracellular fluoresence intensity of a single cardiae myocyts in the control group and carvedilol group was low. The intracellular fluoresence intensity of a single cardiac myocyte in H 2O 2 group was significantly higher than in the control group 15 minutes after intervention (P
