Influence of macrophages on the cell cycle, VEGF receptor and HOXB2 mRNA expression of vascular endothelium
- VernacularTitle:巨噬细胞对血管内皮细胞周期和VEGF受体、HOXB2 mRNA表达的影响
- Author:
Liang LIU
;
Xiaoqi ZHANG
;
Xusheng LIU
- Publication Type:Journal Article
- Keywords:
macrophage;
vascular endothelium;
coculture
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of regulation of endothelium participating angiogenesis by macrophage. Methods Human umbilical vein cell line (ECV-304) was cultured. When 60% of the ECV-304 cells became confluent, human macrophage cell line (U937) after being stimulated by concanavalin A (Con A) was co-cultured with them. The cells were then divided into four groups, i.e. control group (pure ECV-304 culture), co-culture group of Con A with ECV-304, co-culture group of U937 with ECV-304, and co-culture group of U937 with Con A and ECV-304. After 48 hours of co-culture, the morphology of ECV-304 cells was observed under invert microscope, and the changes in cell cycle of ECV-304 were determined by flow cytometry. In addition, the changes in the expression levels of homeobox (HOX) B2 mRNA and VEGF receptor KDR of endothelia cells were assessed by RT-PCR. Results In ECV-304 co-cultured with Con A and U937 group, inter-endothelial gaps were found to be widened, cell shape became abnormal with obvious processes simulating neurons, and the endothelial S phase was prolonged significantly (P
