The effects of human Th2 cytokine on expression of intercellular adhesive molecules on bovin aortic endothelial cells
- VernacularTitle:人Th2细胞因子对牛主动脉内皮细胞表达黏附分子的影响
- Author:
Gang CHEN
;
Zhiren FU
;
Lianghua WANG
- Publication Type:Journal Article
- Keywords:
Th2 cytokine;
bovine aortic endothelial cells;
intercellular adhesion molecule-1;
E-selectin
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of Th2 cytokine (hIL-4, hIL-10, hIL-13) on the bovine aortic endothelial cells(BAECs) stimulated by tumor necrosis factor alpher(TNF-?). Methods BAECs were activated by TNF-?(4ng/ml), and a varied dose of Th2 cytokine(2, 5, 10, 20 and 40ng/ml) was used to incubate BAECs for 2h before stimulation with TNF-?, and then co-incubate for 6h or 18h. The expression of cellular adhesive molecules on BAECs was examined by the cellular enzyme-linked immunoabsorbent assay (Cell-ELISA), and BAECs viability was determined by MTT method. Results BAECs pre-treated with Th2 cytokine could down-regulate the expression of E-selectin and ICAM-1 induced by TNF-? in a dose-dependent manner(P
