?-GABA inhibits release of soluble amyloid precursor protein from rat hippocampal brain slices
- VernacularTitle:?氨基丁酸抑制大鼠海马脑片可溶性淀粉样前体蛋白的释放
- Author:
Guirong ZHANG
;
Denglian GAO
;
Longguang CAO
;
Min YIN
- Publication Type:Journal Article
- Keywords:
?-GABA;
soluble amyloid precursor protein;
Alzheimer disease;
hippocampus
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of ?-GABA on the release of the soluble amyloid precursor protein(sAPP).Methods:Male Sprague-Dawley rats were decapitated and hippocampus was isolated at4℃,then hippocampal slices were quickly prepared in the medium of Krebs-Ringer buffer at0-4℃by a vibration chopper.After the slices were preincubated in Krebs-Ringer buffer at37℃,they were stimulated with various concentrations of ?-GABA(1.0?10 -9 -1.0?10 3 ?mol/L)in Krebs-Ringer buffer.The supernatant was removed from the solution after centrifugated at15000?g.Quantities of total pro-tein were measured by bicinchoninic acid(BCA)assay.The sAPP released into the incubation medium were assayed by West-ern blot.Results:?-GABA increased sAPP release in the incubated hippocampal brain slices.However,it failed to produce special protein.Conclusion:?-GABA within certain concentration range has inhibitory effects on the sAPP release.[
