DISTRIBUTION OF GFP-POSITIVE NEURONS AND THE COLOCALIZATION WITH PARVALBUMIN IN THE CAUDAL SPINAL TRIGEMINAL NUCLEUS IN THE GAD67-GFP KNOCK-IN MOUSE
- VernacularTitle:谷氨酸脱羧酶67-绿色荧光蛋白基因敲入小鼠三叉神经尾侧亚核内GFP阳性神经元的分布及与小白蛋白的共存
- Author:
Jinlian LI
- Publication Type:Journal Article
- Keywords:
Caudal spinal trigeminal nucleus;
GABA;
GFP;
Parvalbumin;
In situ hybridization;
Immunofluorescence histochemistry;
GAD67-GFP knock-in mouse
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To observe the distribution of GABA-containing neurons revealed by GFP expression and the colocalization of the GFP with parvalbumin(PV) in the caudal spinal trigeminal nucleus(Vc),the glutamate decarboxylase 67-green fluorescence protein(GAD67-GFP) knock-in mice were used in the present study. Methods Double-labeled techniques were used by in situ hybridization combined with immunohistochemistry for GFP and double immunofluorescence histochemistry for GFP and NeuN(neuronal nuclei protein,a neuronal marker) or PV.The stained sections were observed under light microscope and a confocal laser-scanning microscope. Results 1.Over 90% of GFP-positive neuronal cell bodies in the laminae Ⅰ and Ⅱ of the Vc showed hybridization signals for GAD67 mRNA,and that almost all neuronal cell bodies with GAD67 mRNA signals were GFP-positive;2.GFP-positive neurons were mainly distributed in the laminae Ⅰ and Ⅱ of the Vc and the vast majority of them were small neurons.The considerable number of GFP-positive processes and somata were most densely observed in the lamina Ⅱ of the Vc.The proportion of GFP-positive neurons in the NeuN-labeled neurons of the Vc was about 19.4% and 24.3% in laminae Ⅰ and Ⅱ,respectively;3.Double-labeled neurons for GFP/PV are mainly found in laminae Ⅰ and Ⅱ of the Vc.The proportion of GFP/PV double-labeled neurons was about 62.4% and 12.8% of total population of PV-and GFP-positive neurons in laminae Ⅰ-Ⅱ of the Vc,respectively.Conclusion GABAergic neurons are mainly distributed in the laminae Ⅰ and Ⅱ of the Vc which related closely to transmission of the nociceptive primary afferent information,and the majority of PV-positive neurons are GABAergic neurons.
