Study on the model of acute pancreatitis induced by of twice intraperitoneal injection excessive doses of L-arginine in mice
- VernacularTitle:分次大剂量L-精氨酸腹腔内注射致小鼠急性水肿性胰腺炎模型的研究
- Author:
Jishun ZHENG
;
Ken CHEN
;
Hui WANG
;
Zhenhe LIN
- Publication Type:Journal Article
- Keywords:
mouse;
L-arginine;
acute edematous pancreatitis(AEP);
animals/models
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a new, simple, stable and classical experimental model of acute edematous pancreatitis. METHODS Male mice were injected intraperitoneally 2?200 mg?(100 g) -1 body weight of L-arginine in an 1 h interval, as a 20% solution in 0.15 mol?L -1 NaCl. Control mice received the same quantity of 0.15 mol?L -1 NaCl. The mice were killed at 6, 12, 24, 48, 72 h following L-arginine administration. The serum amylase level, wet/dry weight ratio of the pancreas, histologic were assessed. RESULTS The serum level of amylase was significantly elevated at 6 h,reached the peak level at 24 h, normalized at 72 h. Histologic examination revealed interstitial edema and inflammatory cell infiltration reached the peak level at 24 h and decreased at 48,72 h. The wet/dry weight ratio of the pancreas changed in accordance with the interstitial edema. CONCLUSIONS The present study has demonstrated that the administration of excessive doses of arginine induces a new, noninvasive experimental model of acute edematous pancreatitis.
