STUDY ON THE PROTECTIVE EFFECTS OF NUTRIENTS ON THE DAMAGE AND APOPTOSIS INDUCED BY HOMOCYSTEINE
- VernacularTitle:营养素防护同型半胱氨酸致ECV304细胞损伤和凋亡
- Author:
Jian GAO
;
Anna XUE
- Publication Type:Journal Article
- Keywords:
homocysteine;
ECV304;
apoptosis;
folic acid;
VE;
Se
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives: To study the apoptosis induced by homocysteine (Hcy) and the protective effects of folic acid, VE and Se. Methods: Inverted microscopy and MTT test were used to observe the protection of these nutrients on the cytotoxic effect of Hcy. DNA agarose gel electrophoresis and flow cytometry were used to observe the inhibition of these nutrients on the apoptosis induced by Hcy. The level of MDA, the activity of SOD and GSH-Px and the generation of active oxygen in cells were also measured to investigate the mechanism of the protective effects. Results: Significant effects of folic acid, VE and Se on the protection of cell damages and the inhibition of apoptosis induced by Hcy were observed. These protective effects may be the results of decreasing MDA in cells provided by folic acid, VE and Se, increasing the activity of SOD by folic acid, increasing the activity of GSH-Px by folic acid and Se, and decreasing the generation of active oxygen by VE. Conclusions: The protective effects of folic acid, VE and Se on the damage of ECV304 cell from Hcy and the inhibitive effect on apoptosis induced by Hcy might be in different mechanisms.
