Adenovirus-mediated gene transfer of rHSG-1 inhibits proliferation of vascular smooth muscle cells from spontaneously hypertensive rats
- VernacularTitle:腺病毒介导的rHSG-1基因转染对自发性高血压大鼠血管平滑肌细胞增殖的影响
- Author:
Pengfei LI
;
Yanhong GUO
;
Qian LI
;
Pengying YAO
;
Guanghu CHEN
- Publication Type:Journal Article
- Keywords:
Muscle, smooth, vascular;
Cell Division;
Cell Cycle;
Adenoviridae;
Rats
- From:
Journal of Peking University(Health Sciences)
2004;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of rat hyperplasia suppressor gene-1(rHSG-1) on the proliferation of aortic vascular smooth muscle cells(VSMCs) from spontaneously hypertensive rats(SHR). Methods:VSMCs were transfected with an adenoviral vector expressing rHSG-1(Ad5rHSG-1). The effect of rHSG-1 on the proliferation of VSMCs was investigated by cell counting, MTT assay and 3H-thymidine incorporation. We also analyzed the cell-cycle using flow cytometry and detected the expression of p27 Kip1 and p21 Cip1 by Western Blot. Results:The proliferation of VSMCs infected with Ad5rHSG-1 was inhibited,a 40% reduction compared with the control group(P
