Stem cell characteristics of human fetal articular cartilage-derived cells
- VernacularTitle:人胚胎关节软骨来源的间充质干细胞
- Author:
Ying CUI
;
Hong WANG
;
Tianrui XU
;
Xuepei LI
;
Lingson LI
- Publication Type:Journal Article
- Keywords:
Cartilage,articular;
Mesenchymal stem cells;
Cell culture;
Cell differentiation
- From:
Journal of Peking University(Health Sciences)
2004;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To verify the hypothesis that cells with characteristics similar to bone marrow mesenchymal stem cells(MSCs) can be isolated and cultured from human fetal articular cartilage. Methods: Human fetal articular cartilages were harvested from fetuses aborted between 12 and 20 weeks. Cells were grown in monolayer cultures in IMDM medium containing antibiotics, L-glutamine and fetal calf serum. Cells were induced to differentiate into adipocytes, osteoblasts, chondrocytes, and neurons. At various time points, parental and passaged cells were subjected to FACS analysis to determine cell phenotype. Results: We successfully isolated and cultured MSCs from human fetal articular cartilage. These cells had the same morphology, phenotype, and ability to differentiate in vitro as MSCs of bone marrow origin. Conclusion: This study shows that cells with characteristics of MSCs can be isolated and cultured from human fetal articular cartilage.
