Seroreactivities of proteinases of Candida albicans, C. tropicalis, and C. parapsilosis in sera from various Candida species-infected mice.
10.3349/ymj.1997.38.3.178
- Author:
Kyoung Ho LEE
1
;
Woon Seob SHIN
;
Hyun Sook PARK
;
Joo Young PARK
;
Choon Myung KOH
Author Information
1. Department of Microbiology, Yonsei University Wonju College of Medicine, Kangwon-Do, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Candida proteinase;
seroreactivity;
HPLC;
common epitope
- MeSH:
Animal;
Candida/genetics*;
Candida/enzymology*;
Candidiasis/enzymology*;
Endopeptidases/analysis*;
Female;
Mice;
Mice, Inbred ICR;
Species Specificity
- From:Yonsei Medical Journal
1997;38(3):178-186
- CountryRepublic of Korea
- Language:English
-
Abstract:
From the culture filtrates of C. albicans, C. tropicalis and C. parapsilosis, proteinases were purified using a series of chromatographic steps consisting of DEAE-Sepharose, Sephacryl S-200 and size-exclusion HPLC which removed contaminating mannoproteins and extraneous proteins. Anti-Candida proteinase antibodies in sera from mice infected with various Candida species were detected using ELISA for serodiagnosis of candidiasis. Three proteinases were blotted by homologous and heterologous anti-proteinase antisera on Western blot analysis. All sera from six Candida species-infected mice were reactive with proteinases of C. albicans, C. tropicalis, and C. parapsilosis, although C. glabrata, C. guilliermondii, and C. krusei did not secrete proteinase. The seroreactivities of proteinase with sera from mice infected with homologous C. albicans and C. tropicalis were higher than those with sera from heterologous Candida species-infected mice. These results suggest that three proteinases have at least one common epitope, but its application for diagnosis of candidiasis should be considered with limits of specificity.
