Fas (CD95,APO-1) antigen expression and function in rat mast cells
- VernacularTitle:大鼠肥大细胞Fas的表达与功能研究
- Author:
Danrong YANG
;
Qingyu XIU
;
Huanxin HAN
- Publication Type:Journal Article
- Keywords:
mast cells;
antigens, CD95;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the surface expression of Fas Ag on RBL 2H3 and its function. Methods RT PCR and Western blot were used to detect the transfection and expression of Fas in RBL 2H3. Surface expression of Fas Ag was studied by immunochemistry. Apoptosis changes following treatment with anti Fas antibody were analyzed using flow cytometic analysis with annexinⅤ. Results It was successful in amplifying gene of rat Fas Ag, and a band of 32kD was detected by Western blot. The Fas Ag expression on the surface of RBL 2H3 by immunochemistry. RBL 2H3 exhibited apoptosis in response to anti Fas treatment. Conclusion Induction of rat mast cell apoptosis by activation of the Fas pathway provide the mechanism by which the number of mast cells may be regulated in the potential therapeutic strategy for anaphylactic diseases
