Transient expression of BMP12 gene transfer to mesenchymal stem cells
- VernacularTitle:BMP12转染骨髓间充质干细胞的瞬时表达
- Author:
Qiwei WANG
;
Wanshan WANG
;
Yingjie PIAO
- Publication Type:Journal Article
- Keywords:
BMP12;
mesenchymal stem cells;
gene transfer;
green fluorescence protein
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of the committed differentiation of mesenchymal stem cells(MSCs) into tenocytes,the present study was carried out. Method NMP12 gene transfer to MSCs was performed with pEGFP C1 expressing vector by electroporation. Results Green fluorescence protein(GFP) expression was detected as early as 6h postelectroporation, peaked at 12h and could maintain 3 days. And then, GFP expression of some cells was weakened. Conclusion The results of the present study indicated that the successful transfection of MSCs was made, an the committed differentiation of MSCs into tenocytes was practicable
